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MAGicBead cfDNA Isolation Kit, 2mLx50 preps

Home Zymo DNA MAGicBead cfDNA Isolation Kit, 2mLx50 preps
Price$ 721.00
  • SKU: ZD4086
  • Pack Size: 2mLx50
Quantity
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MAGicBead cfDNA Isolation Kit, 2mLx50 preps
Description Specifications

The MAGicBead cfDNA Isolation Kit features a unique, magnetic bead surface chemistry that facilitates unparalleled cfDNA recovery from biofluids. Input volumes can range up to 10 mL of plasma. It is compatible with all open automation platforms and a wide range of sample types (serum, saliva, urine, etc.). This kit features a simple, streamlined extraction process with minimal number of steps and does not require the bead drying step common with other similar procedures. Eluted cfDNA is free of enzymatic inhibitors that can interfere with PCR or other sensitive downstream applications, including Next-Gen Sequencing.

Highest Yield: Robust cfDNA isolation from up to 10 mL plasma w/ unique MAGicBead surface technology
NGS-Ready: High-quality cfDNA from plasma, saliva, urine, etc. is ideal for NGS
Compatible: With all open automation platforms



Research or further manufacturing use only, not for food or drug use.
Elution Volume: ≥ 15 µl MAGicBead cfDNA Elution Buffer.
Fragment Sizes: For plasma samples, DNA fragments will typically display a mono-nucleosomal peak in the 140-170 bp range and a genomic DNA fragments peak > 10 kb. However, other multi-nucleosomal apoptotic DNA fragments may also be present.
Processing Time: 1-1.5 hour (~15 minutes hands-on time).
Purity: Eluted cfDNA is NGS-ready. NOTE: Due to low levels of cfDNA available in cell-free biofluids, a spectrophotometer (e.g., NanoDrop) is not recommended for quantification or purity assessment.
Required Equipment: An open automation platform: KingFisher Flex/Apex (Thermo-Fisher Scientific), Fluent X (Tecan), Microlab STAR (Hamilton), OT-2 (Opentrons) and others. For manual applications, a magnetic stand compatible with conical/centrifuge tubes/plates and a rotator capable of at least 30 rpm are needed.
Sample Compatibility: Plasma samples derived from most blood collection tube types are compatible, including Streck DNA BCT. Plasma samples derived from blood collection tubes with heparin are NOT compatible. Highly viscous biofluids, such as whole saliva and synovial fluids, may have limited compatibility. Refer to Appendix A (Page 6) for more details.
Yield: Typical yields range from 0.5 ng to 20 ng from healthy donor plasma samples. This can range from 0.1 ng to 100 ng depending on the health status of donors.

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